Megakaryocyte polyploidization and proplatelet formation in low-attachment conditions
AC Schlinker, MT Duncan, TA DeLuca… - Biochemical …, 2016 - Elsevier
In vitro-derived platelets (PLTs), which could provide an alternative source of PLTs for
patient transfusions, are formed from polyploid megakaryocytes (MKs) that extend long
cytoplasmic projections, termed proplatelets (proPLTs). In this study, we compared
polyploidization and proPLT formation (PPF) of MKs cultured on surfaces that either promote
or inhibit protein adsorption and subsequent cell adhesion. A megakaryoblastic cell line
exhibited increased polyploidization and arrested PPF on a low-attachment surface. Primary …
patient transfusions, are formed from polyploid megakaryocytes (MKs) that extend long
cytoplasmic projections, termed proplatelets (proPLTs). In this study, we compared
polyploidization and proPLT formation (PPF) of MKs cultured on surfaces that either promote
or inhibit protein adsorption and subsequent cell adhesion. A megakaryoblastic cell line
exhibited increased polyploidization and arrested PPF on a low-attachment surface. Primary …