Generation of CAR-T cells by lentiviral transduction
A Okuma - Mammalian Cell Engineering: Methods and Protocols, 2021 - Springer
Mammalian Cell Engineering: Methods and Protocols, 2021•Springer
CAR-T cell therapy is one of the most successful cell-based therapies. T cells are the most
common cells to be genetically modified for cancer therapy, not only because T cells have
cytotoxicity but also because they are easily cultured ex vivo and genetically modified with
viral vectors. Hence, for nonexperts, T cell engineering is an ideal starting point for
mammalian cell engineering or for development of therapeutics. Here, we have described a
basic procedure for lentiviral transduction of human primary T cells to generate a CAR-T cell …
common cells to be genetically modified for cancer therapy, not only because T cells have
cytotoxicity but also because they are easily cultured ex vivo and genetically modified with
viral vectors. Hence, for nonexperts, T cell engineering is an ideal starting point for
mammalian cell engineering or for development of therapeutics. Here, we have described a
basic procedure for lentiviral transduction of human primary T cells to generate a CAR-T cell …
Abstract
CAR-T cell therapy is one of the most successful cell-based therapies. T cells are the most common cells to be genetically modified for cancer therapy, not only because T cells have cytotoxicity but also because they are easily cultured ex vivo and genetically modified with viral vectors. Hence, for nonexperts, T cell engineering is an ideal starting point for mammalian cell engineering or for development of therapeutics. Here, we have described a basic procedure for lentiviral transduction of human primary T cells to generate a CAR-T cell and assays to confirm CAR expression and function.
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