Visualization of extracellular lining layer of lung alveoli by freeze-etching
P Untersee, J Gil, ER Weibel - Respiration Physiology, 1971 - Elsevier
Previous demonstration of a duplex extracellular lining layer of alveoli on fixed and
embedded lung tissue suffered from the fallacy that (1) the air-liquid interface was disturbed
at one step or another, and (2) the tissue had to be chemically treated. The present study
was undertaken to check on the effect of these methodical inadequacies. Air-filled lung
tissue was quick-frozen in Freon near liquid N 2 temperature, cleaved in high vacuum, the
freeze-etehed fracture surface replicated, and the replicas examined by electron …
embedded lung tissue suffered from the fallacy that (1) the air-liquid interface was disturbed
at one step or another, and (2) the tissue had to be chemically treated. The present study
was undertaken to check on the effect of these methodical inadequacies. Air-filled lung
tissue was quick-frozen in Freon near liquid N 2 temperature, cleaved in high vacuum, the
freeze-etehed fracture surface replicated, and the replicas examined by electron …