Organoid Co-culture methods to capture cancer cell–natural killer cell interactions
IS Chan, AJ Ewald - Natural Killer (NK) Cells: Methods and Protocols, 2022 - Springer
IS Chan, AJ Ewald
Natural Killer (NK) Cells: Methods and Protocols, 2022•SpringerMetastasis is a complex process that has been historically difficult to model in culture. Host
immune responses play critical roles in restraining and promoting metastatic tumor cells.
Here we describe a method of 3D organotypic co-culture of natural killer cells and tumor
organoids to capture interactions between the two cellular populations. These assays can
be used to model key aspects of metastatic biology and to screen for the effectiveness of
agents that stimulate natural killer cell cytotoxicity.
immune responses play critical roles in restraining and promoting metastatic tumor cells.
Here we describe a method of 3D organotypic co-culture of natural killer cells and tumor
organoids to capture interactions between the two cellular populations. These assays can
be used to model key aspects of metastatic biology and to screen for the effectiveness of
agents that stimulate natural killer cell cytotoxicity.
Abstract
Metastasis is a complex process that has been historically difficult to model in culture. Host immune responses play critical roles in restraining and promoting metastatic tumor cells. Here we describe a method of 3D organotypic co-culture of natural killer cells and tumor organoids to capture interactions between the two cellular populations. These assays can be used to model key aspects of metastatic biology and to screen for the effectiveness of agents that stimulate natural killer cell cytotoxicity.
Springer
