[HTML][HTML] Annexin A1, formyl peptide receptor, and NOX1 orchestrate epithelial repair

G Leoni, A Alam, PA Neumann… - The Journal of …, 2012 - Am Soc Clin Investig
G Leoni, A Alam, PA Neumann, JD Lambeth, G Cheng, J McCoy, RS Hilgarth, K Kundu
The Journal of clinical investigation, 2012Am Soc Clin Investig
N-formyl peptide receptors (FPRs) are critical regulators of host defense in phagocytes and
are also expressed in epithelia. FPR signaling and function have been extensively studied
in phagocytes, yet their functional biology in epithelia is poorly understood. We describe a
novel intestinal epithelial FPR signaling pathway that is activated by an endogenous FPR
ligand, annexin A1 (ANXA1), and its cleavage product Ac2-26, which mediate activation of
ROS by an epithelial NADPH oxidase, NOX1. We show that epithelial cell migration was …
N-formyl peptide receptors (FPRs) are critical regulators of host defense in phagocytes and are also expressed in epithelia. FPR signaling and function have been extensively studied in phagocytes, yet their functional biology in epithelia is poorly understood. We describe a novel intestinal epithelial FPR signaling pathway that is activated by an endogenous FPR ligand, annexin A1 (ANXA1), and its cleavage product Ac2-26, which mediate activation of ROS by an epithelial NADPH oxidase, NOX1. We show that epithelial cell migration was regulated by this signaling cascade through oxidative inactivation of the regulatory phosphatases PTEN and PTP-PEST, with consequent activation of focal adhesion kinase (FAK) and paxillin. In vivo studies using intestinal epithelial specific Nox1–/–IEC and AnxA1–/– mice demonstrated defects in intestinal mucosal wound repair, while systemic administration of ANXA1 promoted wound recovery in a NOX1-dependent fashion. Additionally, increased ANXA1 expression was observed in the intestinal epithelium and infiltrating leukocytes in the mucosa of ulcerative colitis patients compared with normal intestinal mucosa. Our findings delineate a novel epithelial FPR1/NOX1-dependent redox signaling pathway that promotes mucosal wound repair.
The Journal of Clinical Investigation