A new closed-tube multiplex real-time PCR to detect eleven superantigens of Streptococcus pyogenes identifies a strain without superantigen activity
M Lintges, S Arlt, P Uciechowski, B Plümäkers… - International Journal of …, 2007 - Elsevier
Superantigens (SAgs) are very potent microbial toxins that are involved in severe diseases
such as necrotizing fasciitis and toxic shock syndrome. There are currently 11 different SAgs
that have been identified from Streptococcus pyogenes. In the present study, two sets of
multiplex PCRs were developed for detection of these 11 SAg genes. The first group
comprises spea1− 3+ 5, spec, speg, spej, spek, and spel. The second group consists of
spea1− 4, speh, spei, spem, ssa, and smez. The presence of Streptococcus pyogenes SAg …
such as necrotizing fasciitis and toxic shock syndrome. There are currently 11 different SAgs
that have been identified from Streptococcus pyogenes. In the present study, two sets of
multiplex PCRs were developed for detection of these 11 SAg genes. The first group
comprises spea1− 3+ 5, spec, speg, spej, spek, and spel. The second group consists of
spea1− 4, speh, spei, spem, ssa, and smez. The presence of Streptococcus pyogenes SAg …