Molecular cloning and characterization of a new multispecific organic anion transporter from rat brain
H Kusuhara, T Sekine, N Utsunomiya-Tate… - Journal of Biological …, 1999 - ASBMB
A cDNA encoding the new member of the multispecific organic anion transporter family,
OAT3, was isolated by the reverse transcription-polymerase chain reaction cloning method.
Degenerate primers were designed based on the sequences conserved among OAT1,
OAT2, and organic cation transporter 1 (OCT1), and reverse transcription-polymerase chain
reaction was performed using rat brain poly (A)+ RNA. The 536-amino acid protein
sequence encoded by OAT3 showed 49, 39, and 36% identity to those of OAT1, OAT2, and …
OAT3, was isolated by the reverse transcription-polymerase chain reaction cloning method.
Degenerate primers were designed based on the sequences conserved among OAT1,
OAT2, and organic cation transporter 1 (OCT1), and reverse transcription-polymerase chain
reaction was performed using rat brain poly (A)+ RNA. The 536-amino acid protein
sequence encoded by OAT3 showed 49, 39, and 36% identity to those of OAT1, OAT2, and …
