Human renal organic anion transporter 4 operates as an asymmetric urate transporter
Y Hagos, D Stein, B Ugele, G Burckhardt… - Journal of the American …, 2007 - journals.lww.com
Human organic anion transporter 4 (hOAT4) is located at the apical membrane of proximal
tubule cells and involved in renal secretion and reabsorption of endogenous substances as
well as many drugs and xenobiotics. This study reevaluated the physiologic role, transport
mode, and driving forces of hOAT4. 6-Carboxyfluorescein (6-CF) uptake into HEK293 cells
that stably expressed hOAT4 was saturable, resulting in a K m of 108 μM. 6-CF as well as [3
H] estrone sulfate ([3 H] ES) accumulation by HEK293-hOAT4 cells were abolished by ES …
tubule cells and involved in renal secretion and reabsorption of endogenous substances as
well as many drugs and xenobiotics. This study reevaluated the physiologic role, transport
mode, and driving forces of hOAT4. 6-Carboxyfluorescein (6-CF) uptake into HEK293 cells
that stably expressed hOAT4 was saturable, resulting in a K m of 108 μM. 6-CF as well as [3
H] estrone sulfate ([3 H] ES) accumulation by HEK293-hOAT4 cells were abolished by ES …
