Prooxidant and antioxidant properties of human serum ultrafiltrates toward LDL: important role of uric acid
RA Patterson, ETM Horsley, DS Leake - Journal of Lipid Research, 2003 - ASBMB
Oxidized LDL is present within atherosclerotic lesions, demonstrating a failure of antioxidant
protection. A normal human serum ultrafiltrate of M r below 500 was prepared as a model for
the low M r components of interstitial fluid, and its effects on LDL oxidation were
investigated. The ultrafiltrate (0.3%, v/v) was a potent antioxidant for native LDL, but was a
strong prooxidant for mildly oxidized LDL when copper, but not a water-soluble azo initiator,
was used to oxidize LDL. Adding a lipid hydroperoxide to native LDL induced the …
protection. A normal human serum ultrafiltrate of M r below 500 was prepared as a model for
the low M r components of interstitial fluid, and its effects on LDL oxidation were
investigated. The ultrafiltrate (0.3%, v/v) was a potent antioxidant for native LDL, but was a
strong prooxidant for mildly oxidized LDL when copper, but not a water-soluble azo initiator,
was used to oxidize LDL. Adding a lipid hydroperoxide to native LDL induced the …
