Surface-active phospholipase A2 in mouse spermatozoa
JK Thakkar, J East, D Seyler, RC Franson - Biochimica et Biophysica Acta …, 1983 - Elsevier
The partial characterization of a calcium-dependent phospholipase A 2 associated with
membranes of mouse sperm is described. Intact and sonicated sperm had comparable
phospholipase A 2 activity which was maximal at pH 8.0 using [1-14 C] oleate-labeled
autoclaved Escherichia coli or 1-[1-14 C] stearoyl-2-acyl-3-sn-glycero-
phosphorylethanolamine as substrates. More than 90% of the activity was sedimented when
the sperm sonicate was centrifuged at 100000× g, indicating that the enzyme is almost …
membranes of mouse sperm is described. Intact and sonicated sperm had comparable
phospholipase A 2 activity which was maximal at pH 8.0 using [1-14 C] oleate-labeled
autoclaved Escherichia coli or 1-[1-14 C] stearoyl-2-acyl-3-sn-glycero-
phosphorylethanolamine as substrates. More than 90% of the activity was sedimented when
the sperm sonicate was centrifuged at 100000× g, indicating that the enzyme is almost …
