Helicobacter pylori urease activity is toxic to human gastric epithelial cells
DT Smoot, HL Mobley, GR Chippendale… - Infection and …, 1990 - Am Soc Microbiol
DT Smoot, HL Mobley, GR Chippendale, JF Lewison, JH Resau
Infection and immunity, 1990•Am Soc MicrobiolA human gastric adenocarcinoma cell line was used to evaluate the contribution of urease
from Helicobacter (formerly Campylobacter) pylori to its cytotoxicity. Gastric cells cultured in
medium supplemented with 20 mM urea were exposed to 5 x 10 (6) CFU of H. pylori per ml
with or without the addition of a urease inhibitor, acetohydroxamic acid. Viabilities of cells
exposed to H. pylori for 2, 24, and 48 h, assessed by incorporation of neutral red dye, were
60, 27, and 16%, respectively; however, the viabilities of cells exposed to both H. pylori and …
from Helicobacter (formerly Campylobacter) pylori to its cytotoxicity. Gastric cells cultured in
medium supplemented with 20 mM urea were exposed to 5 x 10 (6) CFU of H. pylori per ml
with or without the addition of a urease inhibitor, acetohydroxamic acid. Viabilities of cells
exposed to H. pylori for 2, 24, and 48 h, assessed by incorporation of neutral red dye, were
60, 27, and 16%, respectively; however, the viabilities of cells exposed to both H. pylori and …
A human gastric adenocarcinoma cell line was used to evaluate the contribution of urease from Helicobacter (formerly Campylobacter) pylori to its cytotoxicity. Gastric cells cultured in medium supplemented with 20 mM urea were exposed to 5 x 10(6) CFU of H. pylori per ml with or without the addition of a urease inhibitor, acetohydroxamic acid. Viabilities of cells exposed to H. pylori for 2, 24, and 48 h, assessed by incorporation of neutral red dye, were 60, 27, and 16%, respectively; however, the viabilities of cells exposed to both H. pylori and acetohydroxamic acid were 92, 46, and 20% after 2, 24, and 48 h, respectively, (P less than 0.001). Therefore, the urease activity of H. pylori may play an important role in its pathogenicity, and inhibition of this enzyme activity may have therapeutic potential.
American Society for Microbiology