Two photon microscopy (TPM) recently emerged as optical tool for the visualization of
immune processes hundreds of micrometers deep in living tissue and organs. Here we
summarize recent work on exploiting this technology to study brain antigen specific T cells.
These cells are the cause of Experimental Autoimmune Encephalomyelitis (EAE) an
autoimmune disease model of Multiple Sclerosis. TPM studies elucidated the dynamics of
the autoaggressive effector T cells in peripheral immune milieus during preclinical EAE …