Methionine synthase from pig liver
R Banerjee, Z Chen, S Gulati - Methods in enzymology, 1997 - Elsevier
Publisher Summary This chapter discusses methionine synthase from pig liver. Several
methods are available for assaying methionine synthase. Mammalian methionine synthase
serves two major metabolic functions. It recycles homocysteine—a toxic catabolite of S-
adenosylmethionine (AdoMet)—and converts the circulating form of folic acid—5-CH 3-H 4
folate—to H 4 folate, which can then support a variety of cellular reactions. The activity of the
enzyme is dependent on the presence of the cofactor, cobalamin. Under in vitro assay …
methods are available for assaying methionine synthase. Mammalian methionine synthase
serves two major metabolic functions. It recycles homocysteine—a toxic catabolite of S-
adenosylmethionine (AdoMet)—and converts the circulating form of folic acid—5-CH 3-H 4
folate—to H 4 folate, which can then support a variety of cellular reactions. The activity of the
enzyme is dependent on the presence of the cofactor, cobalamin. Under in vitro assay …
