MAPK3/1 (ERK1/2) in ovarian granulosa cells are essential for female fertility
HY Fan, Z Liu, M Shimada, E Sterneck, PF Johnson… - Science, 2009 - science.org
HY Fan, Z Liu, M Shimada, E Sterneck, PF Johnson, SM Hedrick, JAS Richards
Science, 2009•science.orgA surge of luteinizing hormone (LH) from the pituitary gland triggers ovulation, oocyte
maturation, and luteinization for successful reproduction in mammals. Because the signaling
molecules RAS and ERK1/2 (extracellular signal–regulated kinases 1 and 2) are activated
by an LH surge in granulosa cells of preovulatory follicles, we disrupted Erk1/2 in mouse
granulosa cells and provide in vivo evidence that these kinases are necessary for LH-
induced oocyte resumption of meiosis, ovulation, and luteinization. In addition, biochemical …
maturation, and luteinization for successful reproduction in mammals. Because the signaling
molecules RAS and ERK1/2 (extracellular signal–regulated kinases 1 and 2) are activated
by an LH surge in granulosa cells of preovulatory follicles, we disrupted Erk1/2 in mouse
granulosa cells and provide in vivo evidence that these kinases are necessary for LH-
induced oocyte resumption of meiosis, ovulation, and luteinization. In addition, biochemical …
A surge of luteinizing hormone (LH) from the pituitary gland triggers ovulation, oocyte maturation, and luteinization for successful reproduction in mammals. Because the signaling molecules RAS and ERK1/2 (extracellular signal–regulated kinases 1 and 2) are activated by an LH surge in granulosa cells of preovulatory follicles, we disrupted Erk1/2 in mouse granulosa cells and provide in vivo evidence that these kinases are necessary for LH-induced oocyte resumption of meiosis, ovulation, and luteinization. In addition, biochemical analyses and selected disruption of the Cebpb gene in granulosa cells demonstrate that C/EBPβ (CCAAT/Enhancer-binding protein–β) is a critical downstream mediator of ERK1/2 activation. Thus, ERK1/2 and C/EBPβ constitute an in vivo LH-regulated signaling pathway that controls ovulation- and luteinization-related events.
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