[HTML][HTML] Toward a human blood serum proteome: analysis by multidimensional separation coupled with mass spectrometry
Blood serum is a complex body fluid that contains various proteins ranging in concentration
over at least 9 orders of magnitude. Using a combination of mass spectrometry technologies
with improvements in sample preparation, we have performed a proteomic analysis with
submilliliter quantities of serum and increased the measurable concentration range for
proteins in blood serum beyond previous reports. We have detected 490 proteins in serum
by on-line reversed-phase microcapillary liquid chromatography coupled with ion trap mass …
over at least 9 orders of magnitude. Using a combination of mass spectrometry technologies
with improvements in sample preparation, we have performed a proteomic analysis with
submilliliter quantities of serum and increased the measurable concentration range for
proteins in blood serum beyond previous reports. We have detected 490 proteins in serum
by on-line reversed-phase microcapillary liquid chromatography coupled with ion trap mass …
